5Z10

Structure of the mechanosensitive Piezo1 channel

Summary for 5Z10

• Entry DOI: 10.2210/pdb5z10/pdb
• EMDB information: 6865
• Descriptor: Piezo-type mechanosensitive ion channel component 1 (1 entity in total)
• Functional Keywords: piezo1, piezo2, mechanogating, channel, membrane protein
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 3
• Total formula weight: 876961.97

Authors

Zhao, Q.,Zhou, H.,Chi, S.,Wang, Y.,Wang, J.,Geng, J.,Wu, K.,Liu, W.,Zhang, T.,Dong, M.-Q.,Wang, J.,Li, X.,Xiao, B. (deposition date: 2017-12-22, release date: 2018-01-31, Last modification date: 2024-11-13)

Primary citation

Zhao, Q.,Zhou, H.,Chi, S.,Wang, Y.,Wang, J.,Geng, J.,Wu, K.,Liu, W.,Zhang, T.,Dong, M.Q.,Wang, J.,Li, X.,Xiao, B.
Structure and mechanogating mechanism of the Piezo1 channel.
Nature, 554:487-492, 2018

PubMed Abstract: The mechanosensitive Piezo channels function as key eukaryotic mechanotransducers. However, their structures and mechanogating mechanisms remain unknown. Here we determine the three-bladed, propeller-like electron cryo-microscopy structure of mouse Piezo1 and functionally reveal its mechanotransduction components. Despite the lack of sequence repetition, we identify nine repetitive units consisting of four transmembrane helices each-which we term transmembrane helical units (THUs)-which assemble into a highly curved blade-like structure. The last transmembrane helix encloses a hydrophobic pore, followed by three intracellular fenestration sites and side portals that contain pore-property-determining residues. The central region forms a 90 Å-long intracellular beam-like structure, which undergoes a lever-like motion to connect THUs to the pore via the interfaces of the C-terminal domain, the anchor-resembling domain and the outer helix. Deleting extracellular loops in the distal THUs or mutating single residues in the beam impairs the mechanical activation of Piezo1. Overall, Piezo1 possesses a unique 38-transmembrane-helix topology and designated mechanotransduction components, which enable a lever-like mechanogating mechanism.

PubMed: 29469092
DOI: 10.1038/nature25743

Experimental method

ELECTRON MICROSCOPY (3.97 Å)