5YUN

Crystal structure of SSB complexed with myc

5YUN の概要

• エントリーDOI: 10.2210/pdb5yun/pdb
• 分子名称: Single-stranded DNA-binding protein, 3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE (3 entities in total)
• 機能のキーワード: single-strand dna binding protein, dna binding protein
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 55733.83

構造登録者

Huang, Y.H.,Huang, C.Y. (登録日: 2017-11-22, 公開日: 2018-10-10, 最終更新日: 2023-11-22)

主引用文献

Huang, C.Y.
Crystal structure of SSB complexed with inhibitor myricetin.
Biochem. Biophys. Res. Commun., 504:704-708, 2018

PubMed Abstract: Single-stranded DNA-binding protein (SSB) is essential for all DNA-dependent cellular processes. SSB inhibitors have been recently suggested as broad-spectrum antibacterial agents in antibiotic development. In this paper, we report the first inhibitor-complexed crystal structure of SSB from Pseudomonas aeruginosa PAO1 (PaSSB) at 2.68 Å resolution (PDB entry 5YUN). The inhibitor, myricetin, is a flavonol that possesses many pharmacological activities, such as anticancer, anti-inflammatory, and antibacterial properties, and is beneficial for humans. Four monomers of PaSSB and two of myricetins were found per asymmetric unit. Various interactions between myricetin and PaSSB were examined. Among these, four residues in PaSSB, Lys7, Arg62, Glu80, and Gly107 were found crucial for forming hydrogen bond to myricetin. These two myricetins occupy the grooves for ssDNA-binding of SSB that may prevent ssDNA-wrapping and ssDNA-binding stably from SSB. In addition to explaining how SSB can be inhibited, the myricetin-SSB interaction modes in this paper may also provide insights into how myricetin can bind and inhibit proteins on cancer-signaling pathways.

PubMed: 30213633
DOI: 10.1016/j.bbrc.2018.08.188

実験手法

X-RAY DIFFRACTION (2.67 Å)