5YUC

Crystal structure of voltage-gated sodium channel NavAb N49K mutant

5YUC の概要

• エントリーDOI: 10.2210/pdb5yuc/pdb
• 分子名称: Ion transport protein, CALCIUM ION, DODECYL-BETA-D-MALTOSIDE, ... (4 entities in total)
• 機能のキーワード: ion channel, membrane protein
• 由来する生物種: Arcobacter butzleri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32579.86

構造登録者

Irie, K.,Shimomura, T.,Fujiyoshi, Y. (登録日: 2017-11-21, 公開日: 2017-12-27, 最終更新日: 2023-11-22)

主引用文献

Irie, K.,Haga, Y.,Shimomura, T.,Fujiyoshi, Y.
Structural insight on the voltage dependence of prokaryotic voltage gated sodium channel NavAb.
FEBS Lett., 2017

PubMed Abstract: Voltage-gated sodium channels are crucial for electro-signalling in living systems. Analysis of the molecular mechanism requires both fine electrophysiological evaluation and high-resolution channel structures. Here, we optimized a dual expression system of NavAb, which is a well-established standard of prokaryotic voltage-gated sodium channels, for E. coli and insect cells using a single plasmid vector to analyse high-resolution protein structures and measure large ionic currents. Using this expression system, we evaluated the voltage dependence and determined the crystal structures of NavAb wild-type and two mutants, E32Q and N49K, whose voltage dependence were positively shifted and essential interactions were lost in voltage sensor domain. The structural and functional comparison elucidated the molecular mechanisms of the voltage dependence of prokaryotic voltage-gated sodium channels.

PubMed: 29274127
DOI: 10.1002/1873-3468.12955

実験手法

X-RAY DIFFRACTION (3.40099156989 Å)