5YT6

Crystal structure of TAX1BP1 UBZ2 in complex with mono-ubiquitin

5YT6 の概要

• エントリーDOI: 10.2210/pdb5yt6/pdb
• 分子名称: Ubiquitin, Tax1-binding protein 1, ZINC ION, ... (7 entities in total)
• 機能のキーワード: tax1bp1, ubiquitin, complex, autophagy receptor, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 52522.55

構造登録者

Pan, L.,Hu, S. (登録日: 2017-11-17, 公開日: 2018-07-11, 最終更新日: 2023-11-22)

主引用文献

Hu, S.,Wang, Y.,Gong, Y.,Liu, J.,Li, Y.,Pan, L.
Mechanistic Insights into Recognitions of Ubiquitin and Myosin VI by Autophagy Receptor TAX1BP1.
J. Mol. Biol., 430:3283-3296, 2018

PubMed Abstract: TAX1BP1, a ubiquitin-binding adaptor, plays critical roles in the innate immunity and selective autophagy. During autophagy, TAX1BP1 may not only function as an autophagy receptor to recruit ubiquitylated substrates for autophagic degradation, but also serve as a Myosin VI cargo adaptor protein for mediating the maturation of autophagosome. However, the mechanistic basis underlying the specific interactions of TAX1BP1 with ubiquitin and Myosin VI remains elusive. Here, using biochemical, NMR and structural analyses, we elucidate the detailed binding mechanism and uncover the key determinants for the interaction between TAX1BP1 and ubiquitin. In addition, we reveal that both tandem zinc-fingers of TAX1BP1 and the conformational rigidity between them are required for the Myosin VI binding of TAX1BP1, and ubiquitin and Myosin VI are mutually exclusive in binding to TAX1BP1. Collectively, our findings provide mechanistic insights into the dual functions of TAX1BP1 in selective autophagy.

PubMed: 29940186
DOI: 10.1016/j.jmb.2018.06.030

実験手法

X-RAY DIFFRACTION (1.501 Å)