5YSM

Crystal Structure Analysis of Rif16

5YSM の概要

• エントリーDOI: 10.2210/pdb5ysm/pdb
• 分子名称: Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: cytochrome p450, rifamycin, metal binding protein, oxidoreductase
• 由来する生物種: Amycolatopsis mediterranei (strain U-32)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49401.87

構造登録者

Li, F.W.,Qi, F.F.,Xiao, Y.L.,Zhao, G.P.,Li, S.Y. (登録日: 2017-11-14, 公開日: 2018-07-04, 最終更新日: 2024-03-27)

主引用文献

Qi, F.,Lei, C.,Li, F.,Zhang, X.,Wang, J.,Zhang, W.,Fan, Z.,Li, W.,Tang, G.L.,Xiao, Y.,Zhao, G.,Li, S.
Deciphering the late steps of rifamycin biosynthesis.
Nat Commun, 9:2342-2342, 2018

PubMed Abstract: Rifamycin-derived drugs, including rifampin, rifabutin, rifapentine, and rifaximin, have long been used as first-line therapies for the treatment of tuberculosis and other deadly infections. However, the late steps leading to the biosynthesis of the industrially important rifamycin SV and B remain largely unknown. Here, we characterize a network of reactions underlying the biosynthesis of rifamycin SV, S, L, O, and B. The two-subunit transketolase Rif15 and the cytochrome P450 enzyme Rif16 are found to mediate, respectively, a unique C-O bond formation in rifamycin L and an atypical P450 ester-to-ether transformation from rifamycin L to B. Both reactions showcase interesting chemistries for these two widespread and well-studied enzyme families.

PubMed: 29904078
DOI: 10.1038/s41467-018-04772-x

実験手法

X-RAY DIFFRACTION (1.9 Å)