5YO8

Crystal structure of beta-C25/C30/C35-prene synthase

5YO8 の概要

• エントリーDOI: 10.2210/pdb5yo8/pdb
• 分子名称: Tetraprenyl-beta-curcumene synthase (2 entities in total)
• 機能のキーワード: terpene synthase, lyase
• 由来する生物種: Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83759.33

構造登録者

Fujihashi, M.,Miki, K. (登録日: 2017-10-27, 公開日: 2018-05-09, 最終更新日: 2024-03-27)

主引用文献

Fujihashi, M.,Sato, T.,Tanaka, Y.,Yamamoto, D.,Nishi, T.,Ueda, D.,Murakami, M.,Yasuno, Y.,Sekihara, A.,Fuku, K.,Shinada, T.,Miki, K.
Crystal structure and functional analysis of large-terpene synthases belonging to a newly found subclass.
Chem Sci, 9:3754-3758, 2018

PubMed Abstract: Thousands of terpenes have been identified to date. However, only two classes of enzymes are known to be involved in their biosynthesis, and each class has characteristic amino-acid motifs. We recently identified a novel large-terpene (C/C/C) synthase, which shares no motifs with known enzymes. To elucidate the molecular mechanism of this enzyme, we determined the crystal structure of a large-β-prene synthase from (BalTS). Surprisingly, the overall structure of BalTS is similar to that of the α-domain of class I terpene synthases although their primary structures are totally different from each other. Two novel aspartate-rich motifs, DYLDNLxD and DY(F,L,W)IDxxED, are identified, and mutations of any one of the aspartates eliminate its enzymatic activity. The present work leads us to propose a new subclass of terpene synthases, class IB, which is probably responsible for large-terpene biosynthesis.

PubMed: 29780507
DOI: 10.1039/c8sc00289d

実験手法

X-RAY DIFFRACTION (1.64 Å)