5YKL

Antimicrobial peptide AY1C designed from the skin secretion of Chinese Odorous frogs

5YKL の概要

• エントリーDOI: 10.2210/pdb5ykl/pdb
• NMR情報: BMRB: 36125
• 分子名称: designed AY1C (1 entity in total)
• 機能のキーワード: cyana 2.1, antimicrobial protein
• 由来する生物種: Odorrana andersonii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2265.87

構造登録者

Pal, I.,Atreya, H.S.,Bhunia, A. (登録日: 2017-10-15, 公開日: 2017-10-25, 最終更新日: 2024-05-15)

主引用文献

Pal, I.,Bhattacharyya, D.,Kar, R.K.,Zarena, D.,Bhunia, A.,Atreya, H.S.
A Peptide-Nanoparticle System with Improved Efficacy against Multidrug Resistant Bacteria.
Sci Rep, 9:4485-4485, 2019

PubMed Abstract: The recent rise of multidrug resistant microbial strains requires development of new and novel therapeutic alternatives. In this study, we present a novel antibacterial system that comprises of modified naturally abundant antimicrobial peptides in conjugation with silver nanoparticles. Further, we propose a simple route to incorporate a cysteine residue either at the N- or C-terminal of the parent peptide. Tagging a cysteine residue at the terminals not only enhances the binding propensity of the resultant peptide with the silver nanoparticle, but also increases its antimicrobial property against several pathogenic bacterial strains including K. pneumoniae. The minimum inhibitory concentration (MIC) values of the cysteine tagged nanoconjugates were obtained in the range of 5-15 μM compared to 50 μM for peptides devoid of the cysteines. The origin and mechanism of such improved activity of the conjugates were investigated using NMR spectroscopy and molecular dynamics (MD) simulations. The application of C-isotope labelled media to track the metabolic lifecycle of E. coli cells provided further insights into the system. MD simulations showed that pore formation in membrane bilayer is mediated through a hydrophobic collapse mechanism. The design strategy described herein opens up new-avenues for using biocompatible nanomedicines as a potential alternative to conventional antibiotics.

PubMed: 30872680
DOI: 10.1038/s41598-019-41005-7

実験手法

SOLUTION NMR