5YI5

human ferritin mutant - E-helix deletion

Summary for 5YI5

• Entry DOI: 10.2210/pdb5yi5/pdb
• EMDB information: 6714 6830
• Descriptor: Ferritin heavy chain (1 entity in total)
• Functional Keywords: ferritin, cage, drug delivery, transport protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 24
• Total formula weight: 494594.52

Authors

Lee, S.G.,Yoon, H.R.,Ahn, B.J.,Jeong, H.,Hyun, J.,Jung, Y.,Kim, H. (deposition date: 2017-10-02, release date: 2018-02-21, Last modification date: 2024-03-27)

Primary citation

Ahn, B.,Lee, S.G.,Yoon, H.R.,Lee, J.M.,Oh, H.J.,Kim, H.M.,Jung, Y.
Four-fold Channel-Nicked Human Ferritin Nanocages for Active Drug Loading and pH-Responsive Drug Release
Angew. Chem. Int. Ed. Engl., 57:2909-2913, 2018

PubMed Abstract: Human ferritins are emerging platforms for non-toxic protein-based drug delivery, owing to their intrinsic or acquirable targeting abilities to cancer cells and hollow cage structures for drug loading. However, reliable strategies for high-level drug encapsulation within ferritin cavities and prompt cellular drug release are still lacking. Ferritin nanocages were developed with partially opened hydrophobic channels, which provide stable routes for spontaneous and highly accumulated loading of Fe -conjugated drugs as well as pH-responsive rapid drug release at endoplasmic pH. Multiple cancer-related compounds, such as doxorubicin, curcumin, and quercetin, were actively and heavily loaded onto the prepared nicked ferritin. Drugs on these minimally modified ferritins were effectively delivered inside cancer cells with high toxicity.

PubMed: 29359486
DOI: 10.1002/anie.201800516

Experimental method

ELECTRON MICROSCOPY (3 Å)