5YEY

The structure of a chair-type G-quadruplex of the human telomeric variant in K+ solution

Summary for 5YEY

• Entry DOI: 10.2210/pdb5yey/pdb
• NMR Information: BMRB: 36116
• Descriptor: DNA (5'-D(*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*TP*GP*GP*G)-3') (1 entity in total)
• Functional Keywords: g-quadruplex, dna
• Biological source: Homo sapiens
• Total number of polymer chains: 1
• Total formula weight: 6661.28

Authors

Liu, C.,Zhou, B.,Kuryavyi, V.V.,Zhu, G. (deposition date: 2017-09-20, release date: 2018-09-26, Last modification date: 2024-05-15)

Primary citation

Liu, C.,Zhou, B.,Geng, Y.,Yan Tam, D.,Feng, R.,Miao, H.,Xu, N.,Shi, X.,You, Y.,Hong, Y.,Tang, B.Z.,Kwan Lo, P.,Kuryavyi, V.,Zhu, G.
A chair-type G-quadruplex structure formed by a human telomeric variant DNA in K+solution.
Chem Sci, 10:218-226, 2019

PubMed Abstract: Guanine tracts of human telomeric DNA sequences are known to fold into eight different four-stranded structures that vary by the conformation of guanine nucleotides arranged in the stack of G-tetrads in their core and by different kinds and orders of connecting loops, called G-quadruplexes. Here, we present a novel G-quadruplex structure formed in K solution by a human telomeric variant d[(GGGTTA)2GGGTTTGGG], T. This variant DNA is located in the subtelomeric regions of human chromosomes 8, 11, 17, and 19 as well as in the DNase hypersensitive region and in the subcentromeric region of chromosome 5. Interestingly, single A18T substitution that makes T different from the human telomeric sequence results in the formation of a three-layer chair-type G-quadruplex, a fold previously unknown among human telomeric repeats, with two loops interacting through the reverse Watson-Crick A6·T18 base pair. The loops are edgewise; glycosidic conformation of guanines is ··· around each tetrad, and each strand of the core has two antiparallel adjacent strands. Our results expand the repertoire of known G-quadruplex folding topologies and may provide a potential target for structure-based anticancer drug design.

PubMed: 30713633
DOI: 10.1039/c8sc03813a

Experimental method

SOLUTION NMR