5YEJ

Crystal structure of BioQ with its naturel double-stranded DNA operator

Summary for 5YEJ

• Entry DOI: 10.2210/pdb5yej/pdb
• Descriptor: TetR family transcriptional regulator, DNA (5'-D(*AP*CP*CP*TP*GP*AP*AP*CP*AP*CP*CP*GP*TP*TP*CP*AP*AP*GP*T)-3'), DNA (5'-D(*AP*CP*TP*TP*GP*AP*AP*CP*GP*GP*TP*GP*TP*TP*CP*AP*GP*GP*T)-3'), ... (4 entities in total)
• Functional Keywords: bioq, dna complex, tetr family, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Mycobacterium smegmatis; More
• Total number of polymer chains: 6
• Total formula weight: 92948.14

Authors

Yan, L.,Guan, Z.Y.,Zou, T.T. (deposition date: 2017-09-17, release date: 2018-09-26, Last modification date: 2024-03-27)

Primary citation

Yan, L.,Tang, Q.,Guan, Z.,Pei, K.,Zou, T.,He, J.
Structural insights into operator recognition by BioQ in the Mycobacterium smegmatis biotin synthesis pathway.
Biochim Biophys Acta Gen Subj, 1862:1843-1851, 2018

PubMed Abstract: Biotin is an essential cofactor in living organisms. The TetR family transcriptional regulator (TFTR) BioQ is the main regulator of biotin synthesis in Mycobacterium smegmatis. BioQ represses the expression of its target genes by binding to a conserved palindromic DNA sequence (the BioQ operator). However, the mechanism by which BioQ recognizes this DNA element has not yet been fully elucidated.

PubMed: 29852200
DOI: 10.1016/j.bbagen.2018.05.015

Experimental method

X-RAY DIFFRACTION (2.698 Å)