5YEJ

Crystal structure of BioQ with its naturel double-stranded DNA operator

5YEJ の概要

• エントリーDOI: 10.2210/pdb5yej/pdb
• 分子名称: TetR family transcriptional regulator, DNA (5'-D(*AP*CP*CP*TP*GP*AP*AP*CP*AP*CP*CP*GP*TP*TP*CP*AP*AP*GP*T)-3'), DNA (5'-D(*AP*CP*TP*TP*GP*AP*AP*CP*GP*GP*TP*GP*TP*TP*CP*AP*GP*GP*T)-3'), ... (4 entities in total)
• 機能のキーワード: bioq, dna complex, tetr family, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mycobacterium smegmatis; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 92948.14

構造登録者

Yan, L.,Guan, Z.Y.,Zou, T.T. (登録日: 2017-09-17, 公開日: 2018-09-26, 最終更新日: 2024-03-27)

主引用文献

Yan, L.,Tang, Q.,Guan, Z.,Pei, K.,Zou, T.,He, J.
Structural insights into operator recognition by BioQ in the Mycobacterium smegmatis biotin synthesis pathway.
Biochim Biophys Acta Gen Subj, 1862:1843-1851, 2018

PubMed Abstract: Biotin is an essential cofactor in living organisms. The TetR family transcriptional regulator (TFTR) BioQ is the main regulator of biotin synthesis in Mycobacterium smegmatis. BioQ represses the expression of its target genes by binding to a conserved palindromic DNA sequence (the BioQ operator). However, the mechanism by which BioQ recognizes this DNA element has not yet been fully elucidated.

PubMed: 29852200
DOI: 10.1016/j.bbagen.2018.05.015

実験手法

X-RAY DIFFRACTION (2.698 Å)