5YDA

The crystal structure of the Acyl Transferase domain of SpnD

5YDA の概要

• エントリーDOI: 10.2210/pdb5yda/pdb
• 分子名称: PKS (2 entities in total)
• 機能のキーワード: pks, acyl transferase domain, biosynthetic protein
• 由来する生物種: Streptomyces sp. CNQ431
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46248.44

構造登録者

Li, Y.,Qu, X.D.,Zhou, J.H. (登録日: 2017-09-12, 公開日: 2018-05-23, 最終更新日: 2024-03-27)

主引用文献

Li, Y.,Zhang, W.,Zhang, H.,Tian, W.Y.,Wu, L.,Wang, S.W.,Zheng, M.M.,Zhang, J.R.,Sun, C.H.,Deng, Z.X.,Sun, Y.H.,Qu, X.D.,Zhou, J.H.
Structural Basis of a Broadly Selective Acyltransferase from the Polyketide Synthase of Splenocin.
Angew. Chem. Int. Ed. Engl., 57:5823-5827, 2018

PubMed Abstract: Polyketides are a large family of pharmaceutically important natural products, and the structural modification of their scaffolds is significant for drug development. Herein, we report high-resolution X-ray crystal structures of the broadly selective acyltransferase (AT) from the splenocin polyketide synthase (SpnD-AT) in the apo form and in complex with benzylmalonyl and pentynylmalonyl extender unit mimics. These structures revealed the molecular basis for the stereoselectivity and substrate specificity of SpnD-AT, and enabled the engineering of the industrially important Ery-AT6 to broaden its substrate scope to include three new types of extender units.

PubMed: 29536601
DOI: 10.1002/anie.201802805

実験手法

X-RAY DIFFRACTION (2.353 Å)