5Y61

YfiB-YfiR complexed with GMP

5Y61 の概要

• エントリーDOI: 10.2210/pdb5y61/pdb
• 分子名称: YfiR, YfiB, GUANOSINE-5'-MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: yfib-yfir complex, gmp, biofilm, signaling protein
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65377.95

構造登録者

Zhou, L.,Xu, M.,Jiang, T. (登録日: 2017-08-10, 公開日: 2017-09-20, 最終更新日: 2024-11-13)

主引用文献

Zhou, L.,Xu, M.,Jiang, T.
Structural insights into the functional role of GMP in modulating the YfiBNR system
Biochem. Biophys. Res. Commun., 493:637-642, 2017

PubMed Abstract: YfiBNR, a tripartite cyclic-di-GMP (c-di-GMP) signalling system, plays an important role in biofilm formation of the gram-negative bacterium P. aeruginosa, which regulates the cellular processes strongly associated with chronic lung infections and drug resistance. The outer-membrane lipoprotein YfiB can release the inhibition of the inner membrane protein YfiN by sequestering the periplasmatic protein YfiR, resulting in the activation of diguanylate cyclase activity of YfiN and the production of c-di-GMP. In contrast to the extensive studies on c-di-GMP, little is known about how GMP acts in the YfiBNR system. Here, we report the crystal structures of YfiR complexed with GMP and YfiB-YfiR complexed with GMP. In the YfiR-GMP complex, GMP is located in a hydrophilic pocket formed by R175/H177/R60, while in the YfiB-YfiR-GMP complex, GMP is located in a slightly separated hydrophilic pocket, with GMP forming hydrogen bonds with both YfiB and YfiR. A binding affinity test and biofilm formation assay indicated that GMP may activate diguanylate cyclase activity to promote biofilm production by promoting the affinity of YfiB and YfiR. This finding thus provides a new perspective for preventing biofilm-related antibiotic resistance and chronic infections.

PubMed: 28870806
DOI: 10.1016/j.bbrc.2017.08.139

実験手法

X-RAY DIFFRACTION (2.99 Å)