5XXU

Small subunit of Toxoplasma gondii ribosome

5XXU の概要

• エントリーDOI: 10.2210/pdb5xxu/pdb
• EMDBエントリー: 6778 6780
• 分子名称: 18S RNA, Ribosomal protein eS8, Ribosomal protein uS4, ... (34 entities in total)
• 機能のキーワード: toxoplasma gondii ribosome, rrna, rprotein, ribosome
• 由来する生物種: Toxoplasma gondii; 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 1177113.34

構造登録者

LI, Z.,Guo, Q.,Zheng, L.,Ji, Y.,Xie, Y.,Lai, D.,Lun, Z.,Suo, X.,Gao, N. (登録日: 2017-07-05, 公開日: 2017-08-30, 最終更新日: 2024-10-09)

主引用文献

Li, Z.,Guo, Q.,Zheng, L.,Ji, Y.,Xie, Y.T.,Lai, D.H.,Lun, Z.R.,Suo, X.,Gao, N.
Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii
Cell Res., 27:1275-1288, 2017

PubMed Abstract: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.

PubMed: 28809395
DOI: 10.1038/cr.2017.104

実験手法

ELECTRON MICROSCOPY (3.35 Å)