5XXO

Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose

5XXO の概要

• エントリーDOI: 10.2210/pdb5xxo/pdb
• 分子名称: Periplasmic beta-glucosidase, beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: glycoside hydrolase family 3, beta-glucosidase, hydrolase
• 由来する生物種: Bacteroides thetaiotaomicron VPI-5482
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 169068.66

構造登録者

Nakajima, M.,Ishiguro, R.,Tanaka, N.,Abe, K.,Maeda, T.,Miyanaga, A.,Takahash, Y.,Sugimoto, N.,Nakai, H.,Taguchi, H. (登録日: 2017-07-04, 公開日: 2017-12-13, 最終更新日: 2023-11-22)

主引用文献

Ishiguro, R.,Tanaka, N.,Abe, K.,Nakajima, M.,Maeda, T.,Miyanaga, A.,Takahashi, Y.,Sugimoto, N.,Nakai, H.,Taguchi, H.
Function and structure relationships of a beta-1,2-glucooligosaccharide-degrading beta-glucosidase.
FEBS Lett., 591:3926-3936, 2017

PubMed Abstract: BT_3567 protein, a putative β-glucosidase from Bacteroides thetaiotaomicron, exhibits higher activity toward Sop (Sop , n: degree of polymerization of β-1,2-glucooligosaccharides) than toward Sop , unlike a known β-glucosidase from Listeria innocua which predominantly prefers Sop . In the complex structure determined by soaking of a D286N mutant crystal with Sop , a Sop moiety was observed at subsites -1 to +2. The glucose moiety at subsite +2 forms a hydrogen bond with Asn81, which is replaced with Gly in the L. innocua β-glucosidase. The K values of the N81G mutant for Sop are much higher than those of the wild-type, suggesting that Asn81 contributes to the binding to substrates longer than Sop .

PubMed: 29131329
DOI: 10.1002/1873-3468.12911

実験手法

X-RAY DIFFRACTION (2.02 Å)