5XU6

Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) from Cryptococcus neoformans

5XU6 の概要

• エントリーDOI: 10.2210/pdb5xu6/pdb
• 分子名称: Inositol-pentakisphosphate 2-kinase, SULFATE ION (3 entities in total)
• 機能のキーワード: inositol 1, 3, 4, 5, 6-pentakisphosphate 2-kinase, ipk1, inositol phosphate kinase, transferase
• 由来する生物種: Cryptococcus neoformans var. grubii serotype A
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 188075.93

構造登録者

Oh, J.,Rhee, S. (登録日: 2017-06-22, 公開日: 2017-10-04, 最終更新日: 2024-11-13)

主引用文献

Oh, J.,Lee, D.G.,Bahn, Y.S.,Rhee, S.
Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from Cryptococcus neoformans.
J. Struct. Biol., 200:118-123, 2017

PubMed Abstract: The fungal pathogen Cryptococcus neoformans is a causative agent of meningoencephalitis in humans. For its pathogenicity, the inositol polyphosphate biosynthetic pathway plays critical roles. Recently, Ipk1 from C. neoformans (CnIpk1) was identified as an inositol 1,3,4,5,6-pentakisphosphate 2-kinase that catalyzes the phosphorylation of IP to form IP, a substrate for subsequent reaction to produce inositol pyrophosphates, such as PP-IP/IP. Furthermore, it was shown that deletion of IPK1 significantly reduces the virulence of C. neoformans, indicating that Ipk1 is a major virulence contributor. In this study, we determined a crystal structure of the apo-form of CnIpk1 at 2.35Å resolution, the first structure for a fungal Ipk1, using a single-wavelength anomalous dispersion method. Even with a low sequence similarity of 26-28%, its overall structure resembles two other Ipk1 orthologs from Arabidopsis thaliana (AtIpk1) and Mus musculus (MmIpk1), and the most crucial residues in the active site are conserved. Unlike AtIpk1 and MmIpk1, however, metal-binding sites for structural stabilization and conformational variations are absent in CnIpk1. The binding environments for substrate IP could be inferred by the two different binding sites for sulfate ion in CnIpk1. Taken together, these observations suggest structural similarities and discrepancies for fungal Ipk1 among members of the Ipk1 family and provide structural information for the possible development of drug design for treatment of cryptococcosis.

PubMed: 28919350
DOI: 10.1016/j.jsb.2017.09.004

実験手法

X-RAY DIFFRACTION (2.35 Å)