5XSZ

Crystal structure of zebrafish lysophosphatidic acid receptor LPA6

5XSZ の概要

• エントリーDOI: 10.2210/pdb5xsz/pdb
• 分子名称: Lysophosphatidic acid receptor 6a,Endolysin,Lysophosphatidic acid receptor 6a, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (2 entities in total)
• 機能のキーワード: alpha helical, membrane protein
• 由来する生物種: Danio rerio (Zebrafish); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55455.19

構造登録者

Taniguchi, R.,Nishizawa, T.,Ishitani, R.,Nureki, O. (登録日: 2017-06-16, 公開日: 2017-08-16, 最終更新日: 2024-10-23)

主引用文献

Taniguchi, R.,Inoue, A.,Sayama, M.,Uwamizu, A.,Yamashita, K.,Hirata, K.,Yoshida, M.,Tanaka, Y.,Kato, H.E.,Nakada-Nakura, Y.,Otani, Y.,Nishizawa, T.,Doi, T.,Ohwada, T.,Ishitani, R.,Aoki, J.,Nureki, O.
Structural insights into ligand recognition by the lysophosphatidic acid receptor LPA6
Nature, 548:356-360, 2017

PubMed Abstract: Lysophosphatidic acid (LPA) is a bioactive lipid composed of a phosphate group, a glycerol backbone, and a single acyl chain that varies in length and saturation. LPA activates six class A G-protein-coupled receptors to provoke various cellular reactions. Because LPA signalling has been implicated in cancer and fibrosis, the LPA receptors are regarded as promising drug targets. The six LPA receptors are subdivided into the endothelial differentiation gene (EDG) family (LPA-LPA) and the phylogenetically distant non-EDG family (LPA-LPA). The structure of LPA has enhanced our understanding of the EDG family of LPA receptors. By contrast, the functional and pharmacological characteristics of the non-EDG family of LPA receptors have remained unknown, owing to the lack of structural information. Although the non-EDG LPA receptors share sequence similarity with the P2Y family of nucleotide receptors, the LPA recognition mechanism cannot be deduced from the P2Y and P2Y structures because of the large differences in the chemical structures of their ligands. Here we determine the 3.2 Å crystal structure of LPA, the gene deletion of which is responsible for congenital hair loss, to clarify the ligand recognition mechanism of the non-EDG family of LPA receptors. Notably, the ligand-binding pocket of LPA is laterally open towards the membrane, and the acyl chain of the lipid used for the crystallization is bound within this pocket, indicating the binding mode of the LPA acyl chain. Docking and mutagenesis analyses also indicated that the conserved positively charged residues within the central cavity recognize the phosphate head group of LPA by inducing an inward shift of transmembrane helices 6 and 7, suggesting that the receptor activation is triggered by this conformational rearrangement.

PubMed: 28792932
DOI: 10.1038/nature23448

実験手法

X-RAY DIFFRACTION (3.2 Å)