5XLS

Crystal structure of UraA in occluded conformation

5XLS の概要

• エントリーDOI: 10.2210/pdb5xls/pdb
• 分子名称: Uracil permease, URACIL, 12-TUNGSTOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: occluded conformation, dimer, transport protein
• 由来する生物種: Escherichia coli O157:H7
• 細胞内の位置: Cell inner membrane ; Multi- pass membrane protein : P0AGM8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51812.87

構造登録者

Yu, X.Z.,Yang, G.H.,Yan, C.Y.,Yan, N. (登録日: 2017-05-11, 公開日: 2017-07-05, 最終更新日: 2023-11-22)

主引用文献

Yu, X.,Yang, G.,Yan, C.,Baylon, J.L.,Jiang, J.,Fan, H.,Lu, G.,Hasegawa, K.,Okumura, H.,Wang, T.,Tajkhorshid, E.,Li, S.,Yan, N.
Dimeric structure of the uracil:proton symporter UraA provides mechanistic insights into the SLC4/23/26 transporters
Cell Res., 27:1020-1033, 2017

PubMed Abstract: The Escherichia coli uracil:proton symporter UraA is a prototypical member of the nucleobase/ascorbate transporter (NAT) or nucleobase/cation symporter 2 (NCS2) family, which corresponds to the human solute carrier family SLC23. UraA consists of 14 transmembrane segments (TMs) that are organized into two distinct domains, the core domain and the gate domain, a structural fold that is also shared by the SLC4 and SLC26 transporters. Here we present the crystal structure of UraA bound to uracil in an occluded state at 2.5 Å resolution. Structural comparison with the previously reported inward-open UraA reveals pronounced relative motions between the core domain and the gate domain as well as intra-domain rearrangement of the gate domain. The occluded UraA forms a dimer in the structure wherein the gate domains are sandwiched by two core domains. In vitro and in vivo biochemical characterizations show that UraA is at equilibrium between dimer and monomer in all tested detergent micelles, while dimer formation is necessary for the transport activity. Structural comparison between the dimeric UraA and the recently reported inward-facing dimeric UapA provides important insight into the transport mechanism of SLC23 transporters.

PubMed: 28621327
DOI: 10.1038/cr.2017.83

実験手法

X-RAY DIFFRACTION (2.5 Å)