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5XJ6

Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the glycerol 3-phosphate form

5XJ6 の概要
エントリーDOI10.2210/pdb5xj6/pdb
関連するPDBエントリー5XJ5 5XJ7 5XJ8 5XJ9
分子名称Glycerol-3-phosphate acyltransferase, SN-GLYCEROL-3-PHOSPHATE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (5 entities in total)
機能のキーワードglycerol 3-phosphate acyltransferase, glycerylphosphate acyltransferase, gpat, in meso, lipid cubic phase, lipidic cubic phase, lipid metabolism, monoacylglycerol, phospholipid biosynthesis, plsy, substrate, ygih, transferase
由来する生物種Aquifex aeolicus
細胞内の位置Cell inner membrane ; Multi-pass membrane protein : O66905
タンパク質・核酸の鎖数1
化学式量合計23585.21
構造登録者
Li, Z.,Tang, Y.,Li, D. (登録日: 2017-04-30, 公開日: 2017-12-06, 最終更新日: 2024-03-27)
主引用文献Li, Z.,Tang, Y.,Wu, Y.,Zhao, S.,Bao, J.,Luo, Y.,Li, D.
Structural insights into the committed step of bacterial phospholipid biosynthesis.
Nat Commun, 8:1691-1691, 2017
Cited by
PubMed Abstract: The membrane-integral glycerol 3-phosphate (G3P) acyltransferase PlsY catalyses the committed and essential step in bacterial phospholipid biosynthesis by acylation of G3P, forming lysophosphatidic acid. It contains no known acyltransferase motifs, lacks eukaryotic homologs, and uses the unusual acyl-phosphate as acyl donor, as opposed to acyl-CoA or acyl-carrier protein for other acyltransferases. Previous studies have identified several PlsY inhibitors as potential antimicrobials. Here we determine the crystal structure of PlsY at 1.48 Å resolution, revealing a seven-transmembrane helix fold. Four additional substrate- and product-bound structures uncover the atomic details of its relatively inflexible active site. Structure and mutagenesis suggest a different acylation mechanism of 'substrate-assisted catalysis' that, unlike other acyltransferases, does not require a proteinaceous catalytic base to complete. The structure data and a high-throughput enzymatic assay developed in this work should prove useful for virtual and experimental screening of inhibitors against this vital bacterial enzyme.
PubMed: 29167463
DOI: 10.1038/s41467-017-01821-9
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.37 Å)
構造検証レポート
Validation report summary of 5xj6
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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