5XJ1

Crystal structure of spRlmCD

5XJ1 の概要

• エントリーDOI: 10.2210/pdb5xj1/pdb
• 関連するPDBエントリー: 5XJ2
• 分子名称: Uncharacterized RNA methyltransferase SP_1029, GLYCEROL, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: 23s rrna, u747, methyltransferase, transferase
• 由来する生物種: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52091.86

構造登録者

Jiang, Y.,Gong, Q. (登録日: 2017-04-28, 公開日: 2017-11-01, 最終更新日: 2023-11-22)

主引用文献

Jiang, Y.,Li, F.,Wu, J.,Shi, Y.,Gong, Q.
Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD
PLoS ONE, 12:e0185226-e0185226, 2017

PubMed Abstract: RlmCD has recently been identified as the S-adenosyl methionine (SAM)-dependent methyltransferase responsible for the formation of m5U at U747 and U1939 of 23S ribosomal RNA in Streptococcus pneumoniae. In this research, we determine the high-resolution crystal structures of apo-form RlmCD and its complex with SAH. Using an in-vitro methyltransferase assay, we reveal the crucial residues for its catalytic functions. Furthermore, structural comparison between RlmCD and its structural homologue RumA, which only catalyzes the m5U1939 in Escherichia coli, implicates that a unique long linker in the central domain of RlmCD is the key factor in determining its substrate selectivity. Its significance in the enzyme activity of RlmCD is further confirmed by in-vitro methyltransferase assay.

PubMed: 28949991
DOI: 10.1371/journal.pone.0185226

実験手法

X-RAY DIFFRACTION (1.753 Å)