5XIB

Crystal Structure of HasAp with Fe-5,15-Diphenylporphyrin

5XIB の概要

• エントリーDOI: 10.2210/pdb5xib/pdb
• 分子名称: Heme acquisition protein HasAp, 5,15-Diphenylporphyrin containing FE (3 entities in total)
• 機能のキーワード: heme acquisition protein, transport protein
• 由来する生物種: Pseudomonas aeruginosa str. PAO1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38835.82

構造登録者

Shoji, O.,Uehara, H.,Sugimoto, H.,Shiro, Y.,Watanabe, Y. (登録日: 2017-04-26, 公開日: 2017-12-06, 最終更新日: 2023-11-22)

主引用文献

Uehara, H.,Shisaka, Y.,Nishimura, T.,Sugimoto, H.,Shiro, Y.,Miyake, Y.,Shinokubo, H.,Watanabe, Y.,Shoji, O.
Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group
Angew. Chem. Int. Ed. Engl., 56:15279-15283, 2017

PubMed Abstract: Iron(III)-5,15-diphenylporphyrin and several derivatives were accommodated by HasA, a heme acquisition protein secreted by Pseudomonas aeruginosa, despite possessing bulky substituents at the meso position of the porphyrin. Crystal structure analysis revealed that the two phenyl groups at the meso positions of porphyrin extend outside HasA. It was shown that the growth of P. aeruginosa was inhibited in the presence of HasA coordinating the synthetic porphyrins under iron-limiting conditions, and that the structure of the synthetic porphyrins greatly affects the inhibition efficiency.

PubMed: 28921809
DOI: 10.1002/anie.201707212

実験手法

X-RAY DIFFRACTION (2.3 Å)