5XHT

The PHD finger of human Kiaa1045 protein

5XHT の概要

• エントリーDOI: 10.2210/pdb5xht/pdb
• NMR情報: BMRB: 36075
• 分子名称: PHD finger protein 24, ZINC ION (2 entities in total)
• 機能のキーワード: phd finger, zinc finger, metal binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6989.53

構造登録者

Miyamoto, K. (登録日: 2017-04-24, 公開日: 2018-05-02, 最終更新日: 2024-05-01)

主引用文献

Miyamoto, K.,Yamashita, A.,Saito, K.
Solution structure of the PHD finger from the human KIAA1045 protein
Protein Sci., 27:987-992, 2018

PubMed Abstract: Cross-brace structural motifs are required as a scaffold to design artificial RING fingers (ARFs) that function as ubiquitin ligase (E3) in ubiquitination and have specific ubiquitin-conjugating enzyme (E2)-binding capabilities. The Simple Modular Architecture Research Tool database predicted the amino acid sequence 131-190 (KIAA1045ZF) of the human KIAA1045 protein as an unidentified structural region. Herein, the stoichiometry of zinc ions estimated spectrophotometrically by the metallochromic indicator revealed that the KIAA1045ZF motif binds to two zinc atoms. The structure of the KIAA1045ZF motif bound to the zinc atoms was elucidated at the atomic level by nuclear magnetic resonance. The actual structure of the KIAA1045ZF motif adopts a C HC -type PHD fold belonging to the cross-brace structural family. Therefore, the utilization of the KIAA1045ZF motif as a scaffold may lead to the creation of a novel ARF.

PubMed: 29430827
DOI: 10.1002/pro.3389

実験手法

SOLUTION NMR