5XGU

Escherichia coli. RNase R

5XGU の概要

• エントリーDOI: 10.2210/pdb5xgu/pdb
• 分子名称: Ribonuclease R, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: exoribonuclease, hydrolase
• 由来する生物種: Escherichia coli DEC6A
• 細胞内の位置: Cytoplasm : H4USN4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 146721.28

構造登録者

Chu, L.Y.,Hsieh, T.J.,Yuan, H.S. (登録日: 2017-04-17, 公開日: 2017-10-18, 最終更新日: 2023-11-22)

主引用文献

Chu, L.Y.,Hsieh, T.J.,Golzarroshan, B.,Chen, Y.P.,Agrawal, S.,Yuan, H.S.
Structural insights into RNA unwinding and degradation by RNase R.
Nucleic Acids Res., 45:12015-12024, 2017

PubMed Abstract: RNase R is a conserved exoribonuclease in the RNase II family that primarily participates in RNA decay in all kingdoms of life. RNase R degrades duplex RNA with a 3' overhang, suggesting that it has RNA unwinding activity in addition to its 3'-to-5' exoribonuclease activity. However, how RNase R coordinates RNA binding with unwinding to degrade RNA remains elusive. Here, we report the crystal structure of a truncated form of Escherichia coli RNase R (residues 87-725) at a resolution of 1.85 Å. Structural comparisons with other RNase II family proteins reveal two open RNA-binding channels in RNase R and suggest a tri-helix 'wedge' region in the RNB domain that may induce RNA unwinding. We constructed two tri-helix wedge mutants and they indeed lost their RNA unwinding but not RNA binding or degrading activities. Our results suggest that the duplex RNA with an overhang is bound in the two RNA-binding channels in RNase R. The 3' overhang is threaded into the active site and the duplex RNA is unwound upon reaching the wedge region during RNA degradation. Thus, RNase R is a proficient enzyme, capable of concurrently binding, unwinding and degrading structured RNA in a highly processive manner during RNA decay.

PubMed: 29036353
DOI: 10.1093/nar/gkx880

実験手法

X-RAY DIFFRACTION (1.846 Å)