5XG5

Crystal structure of Mitsuba-1 with bound NAcGal

5XG5 の概要

• エントリーDOI: 10.2210/pdb5xg5/pdb
• 分子名称: MITSUBA-1, 2-acetamido-2-deoxy-alpha-D-galactopyranose (3 entities in total)
• 機能のキーワード: protein design, lectin, de novo protein, sugar binding protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17106.25

構造登録者

Terada, D.,Voet, A.R.D.,Kamata, K.,Zhang, K.Y.J.,Tame, J.R.H. (登録日: 2017-04-12, 公開日: 2017-07-12, 最終更新日: 2024-03-27)

主引用文献

Terada, D.,Voet, A.R.D.,Noguchi, H.,Kamata, K.,Ohki, M.,Addy, C.,Fujii, Y.,Yamamoto, D.,Ozeki, Y.,Tame, J.R.H.,Zhang, K.Y.J.
Computational design of a symmetrical beta-trefoil lectin with cancer cell binding activity.
Sci Rep, 7:5943-5943, 2017

PubMed Abstract: Computational protein design has advanced very rapidly over the last decade, but there remain few examples of artificial proteins with direct medical applications. This study describes a new artificial β-trefoil lectin that recognises Burkitt's lymphoma cells, and which was designed with the intention of finding a basis for novel cancer treatments or diagnostics. The new protein, called "Mitsuba", is based on the structure of the natural shellfish lectin MytiLec-1, a member of a small lectin family that uses unique sequence motifs to bind α-D-galactose. The three subdomains of MytiLec-1 each carry one galactose binding site, and the 149-residue protein forms a tight dimer in solution. Mitsuba (meaning "three-leaf" in Japanese) was created by symmetry constraining the structure of a MytiLec-1 subunit, resulting in a 150-residue sequence that contains three identical tandem repeats. Mitsuba-1 was expressed and crystallised to confirm the X-ray structure matches the predicted model. Mitsuba-1 recognises cancer cells that express globotriose (Galα(1,4)Galβ(1,4)Glc) on the surface, but the cytotoxicity is abolished.

PubMed: 28724971
DOI: 10.1038/s41598-017-06332-7

実験手法

X-RAY DIFFRACTION (1.54 Å)