5XG0

Crystal structure of a novel PET hydrolase from Ideonella sakaiensis 201-F6

5XG0 の概要

• エントリーDOI: 10.2210/pdb5xg0/pdb
• 関連するPDBエントリー: 5XFX 5XFY 5XFZ
• 分子名称: Poly(ethylene terephthalate) hydrolase (2 entities in total)
• 機能のキーワード: metal-binding, substrate binding, acidocalcisomal pyrophosphatase, inhibitor, hydrolase
• 由来する生物種: Ideonella sakaiensis (strain 201-F6)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 83600.79

構造登録者

Han, X.,Liu, W.D.,Zheng, Y.Y.,Chen, C.C.,Guo, R.T. (登録日: 2017-04-11, 公開日: 2017-12-20, 最終更新日: 2023-11-22)

主引用文献

Han, X.,Liu, W.,Huang, J.W.,Ma, J.,Zheng, Y.,Ko, T.P.,Xu, L.,Cheng, Y.S.,Chen, C.C.,Guo, R.T.
Structural insight into catalytic mechanism of PET hydrolase
Nat Commun, 8:2106-2106, 2017

PubMed Abstract: PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

PubMed: 29235460
DOI: 10.1038/s41467-017-02255-z

実験手法

X-RAY DIFFRACTION (1.58 Å)