5XFC

Serial femtosecond X-ray structure of a stem domain of human O-mannose beta-1,2-N-acetylglucosaminyltransferase solved by Se-SAD using XFEL (refined against 13,000 patterns)

Summary for 5XFC

• Entry DOI: 10.2210/pdb5xfc/pdb
• Related: 5XFD
• Descriptor: Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1, 4-nitrophenyl beta-D-mannopyranoside (3 entities in total)
• Functional Keywords: glycosyltransferase, carbohydrate-binding domain, sugar binding protein
• Biological source: Homo sapiens (Human)
• Cellular location: Golgi apparatus membrane ; Single-pass type II membrane protein : Q8WZA1
• Total number of polymer chains: 2
• Total formula weight: 36129.91

Authors

Kuwabara, N.,Fumiaki, Y.,Kato, R.,Manya, H. (deposition date: 2017-04-10, release date: 2017-08-30, Last modification date: 2023-11-15)

• Primary citation: Yamashita, K.,Kuwabara, N.,Nakane, T.,Murai, T.,Mizohata, E.,Sugahara, M.,Pan, D.,Masuda, T.,Suzuki, M.,Sato, T.,Kodan, A.,Yamaguchi, T.,Nango, E.,Tanaka, T.,Tono, K.,Joti, Y.,Kameshima, T.,Hatsui, T.,Yabashi, M.,Manya, H.,Endo, T.,Kato, R.,Senda, T.,Kato, H.,Iwata, S.,Ago, H.,Yamamoto, M.,Yumoto, F.,Nakatsu, T.; Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography; IUCrJ, 4:639-647, 2017; PubMed: 28989719; DOI: 10.1107/S2052252517008557

Experimental method

X-RAY DIFFRACTION (1.4 Å)