5XFA

Crystal structure of NAD+-reducing [NiFe]-hydrogenase in the H2-reduced state

5XFA の概要

• エントリーDOI: 10.2210/pdb5xfa/pdb
• 関連するPDBエントリー: 5XF9
• 分子名称: NAD-reducing hydrogenase, IRON/SULFUR CLUSTER, FE2/S2 (INORGANIC) CLUSTER, ... (10 entities in total)
• 機能のキーワード: hydrogenase, ni-fe, fe-s, oxidoreductase
• 由来する生物種: Hydrogenophilus thermoluteolus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 330305.03

構造登録者

Shomura, Y.,Taketa, M.,Nakashima, H.,Tai, H.,Nakagawa, H.,Ikeda, Y.,Ishii, M.,Igarashi, Y.,Nishihara, H.,Yoon, K.S.,Ogo, S.,Hirota, S.,Higuchi, Y. (登録日: 2017-04-09, 公開日: 2017-08-23, 最終更新日: 2023-11-22)

主引用文献

Shomura, Y.,Taketa, M.,Nakashima, H.,Tai, H.,Nakagawa, H.,Ikeda, Y.,Ishii, M.,Igarashi, Y.,Nishihara, H.,Yoon, K.S.,Ogo, S.,Hirota, S.,Higuchi, Y.
Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase
Science, 357:928-932, 2017

PubMed Abstract: NAD (oxidized form of NAD:nicotinamide adenine dinucleotide)-reducing soluble [NiFe]-hydrogenase (SH) is phylogenetically related to NADH (reduced form of NAD):quinone oxidoreductase (complex I), but the geometrical arrangements of the subunits and Fe-S clusters are unclear. Here, we describe the crystal structures of SH in the oxidized and reduced states. The cluster arrangement is similar to that of complex I, but the subunits orientation is not, which supports the hypothesis that subunits evolved as prebuilt modules. The oxidized active site includes a six-coordinate Ni, which is unprecedented for hydrogenases, whose coordination geometry would prevent O from approaching. In the reduced state showing the normal active site structure without a physiological electron acceptor, the flavin mononucleotide cofactor is dissociated, which may be caused by the oxidation state change of nearby Fe-S clusters and may suppress production of reactive oxygen species.

PubMed: 28860386
DOI: 10.1126/science.aan4497

実験手法

X-RAY DIFFRACTION (2.7 Å)