5XEX

Crystal structure of S.aureus PNPase catalytic domain

5XEX の概要

• エントリーDOI: 10.2210/pdb5xex/pdb
• 分子名称: Polyribonucleotide nucleotidyltransferase, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: polynucleotide phosphorylase, catalytic domain, transferase
• 由来する生物種: Staphylococcus aureus (strain NCTC 8325)
• 細胞内の位置: Cytoplasm : Q2FZ20
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 375254.51

構造登録者

Wang, X.,Zhang, X.,Zang, J. (登録日: 2017-04-06, 公開日: 2017-10-18, 最終更新日: 2023-11-22)

主引用文献

Wang, X.,Wang, C.,Wu, M.,Tian, T.,Cheng, T.,Zhang, X.,Zang, J.
Enolase binds to RnpA in competition with PNPase in Staphylococcus aureus
FEBS Lett., 591:3523-3535, 2017

PubMed Abstract: The RNA degradosome of the pathogen Staphylococcus aureus regulates the metabolism of RNA, the expression of virulence factors, and the formation of biofilms. It is composed of the RNases J1/J2, RNase Y, CshA, PNPase, Enolase, Pfk, and a newly identified component, RnpA. However, the function and new partners of RnpA in RNA degradosome remain unknown. Here, we identified PNPase and Enolase as two novel partners for RnpA. Further studies revealed that Enolase interacts with RnpA in competition with PNPase. Enzymatic assays showed that RnpA increases Enolase activity but has no effect on PNPase. These findings provide more information about the functional relationship between RnpA and RNA degradosome.

PubMed: 28960276
DOI: 10.1002/1873-3468.12859

実験手法

X-RAY DIFFRACTION (2.2 Å)