5XEU

crystal structure of Hcp2 from Salmonella typhimurium

5XEU の概要

• エントリーDOI: 10.2210/pdb5xeu/pdb
• 分子名称: Hcp1 family type VI secretion system effector (1 entity in total)
• 機能のキーワード: hexameric, cytosolic protein
• 由来する生物種: Salmonella typhimurium
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 106499.31

構造登録者

Lin, Q.P.,Gao, Z.Q.,Zhang, H. (登録日: 2017-04-06, 公開日: 2017-08-16, 最終更新日: 2023-11-22)

主引用文献

Lin, Q.P.,Gao, Z.Q.,Geng, Z.,Zhang, H.,Dong, Y.H.
Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium
Acta Crystallogr F Struct Biol Commun, 73:463-468, 2017

PubMed Abstract: STM0279 is a putative cytoplasmic protein from Salmonella typhimurium and was recently renamed haemolysin co-regulated protein 2 (Hcp2), with the neighbouring STM0276 being Hcp1. Both of them are encoded by the type VI secretion system (T6SS) of the Salmonella pathogenicity island 6 (SPI-6) locus and have high sequence identity. The Hcp proteins may function as a vital component of the T6SS nanotube and as a transporter and chaperone of diverse effectors from the bacterial T6SS. In this study, the crystal structure and the oligomeric state in solution of Hcp2 from S. typhimurium (StHcp2) were investigated. The crystal structure refined to 3.0 Å resolution showed that the protein is composed of a β-barrel domain with extended loops and can form hexameric rings as observed in known Hcp homologues. Mutation of the extended loop was found to partly destabilize the hexameric conformation into monomers or cause the production of inclusion bodies, suggesting it has an important role in hexameric ring formation.

PubMed: 28777089
DOI: 10.1107/S2053230X17010512

実験手法

X-RAY DIFFRACTION (3 Å)