5XEC

Heterodimer constructed from PA cyt c551-HT cyt c552 and HT cyt c552-PA cyt c551 chimeric proteins

5XEC の概要

• エントリーDOI: 10.2210/pdb5xec/pdb
• 関連するPDBエントリー: 5XED
• 分子名称: Cytochrome c-552,Cytochrome c-551, Cytochrome c-551,Cytochrome c-552, HEME C, ... (4 entities in total)
• 機能のキーワード: chimeric protein, electron transport
• 由来する生物種: Hydrogenobacter thermophilus; 詳細
• 細胞内の位置: Periplasm: P00099
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18527.91

構造登録者

Zhang, M.,Nakanishi, T.,Yamanaka, M.,Nagao, S.,Yanagisawa, S.,Shomura, Y.,Shibata, N.,Ogura, T.,Higuchi, Y.,Hirota, S. (登録日: 2017-04-04, 公開日: 2017-08-09, 最終更新日: 2024-11-13)

主引用文献

Zhang, M.,Nakanishi, T.,Yamanaka, M.,Nagao, S.,Yanagisawa, S.,Shomura, Y.,Shibata, N.,Ogura, T.,Higuchi, Y.,Hirota, S.
Rational Design of Domain-Swapping-Based c-Type Cytochrome Heterodimers by Using Chimeric Proteins.
Chembiochem, 18:1712-1715, 2017

PubMed Abstract: The design of protein oligomers with multiple active sites has been gaining interest, owing to their potential use for biomaterials, which has encouraged researchers to develop a new design method. Three-dimensional domain swapping is the unique phenomenon in which protein molecules exchange the same structural region between each other. Herein, to construct oligomeric heme proteins with different active sites by utilizing domain swapping, two c-type cytochrome-based chimeric proteins have been constructed and the domains swapped. According to X-ray crystallographic analysis, the two chimeric proteins formed a domain-swapped dimer with two His/Met coordinated hemes. By mutating the heme coordination structure of one of the two chimeric proteins, a domainswapped heterodimer with His/Met and His/H O coordinated hemes was formed. Binding of an oxygen molecule to the His/H O site of the heterodimer was confirmed by resonance Raman spectroscopy, in which the Fe-O stretching band was observed at 580 cm for the reduced/oxygenated heterodimer (at 554 cm under an O atmosphere). These results show that domain swapping is a useful method to design multiheme proteins.

PubMed: 28660650
DOI: 10.1002/cbic.201700219

実験手法

X-RAY DIFFRACTION (1.1 Å)