5XDZ

Crystal structure of zebrafish SNX25 PX domain

Summary for 5XDZ

• Entry DOI: 10.2210/pdb5xdz/pdb
• Descriptor: Cellular trafficking protein, SODIUM ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: sorting nexin, px domain, proton transport
• Biological source: Danio rerio (Zebrafish); More
• Total number of polymer chains: 2
• Total formula weight: 27884.16

Authors

Su, K.,Zhang, Y.,Xu, J.,Liu, J. (deposition date: 2017-03-30, release date: 2017-06-14, Last modification date: 2023-11-22)

Primary citation

Su, K.,Xu, T.,Yu, Z.,Zhu, J.,Zhang, Y.,Wu, M.,Xiong, Y.,Liu, J.,Xu, J.
Structure of the PX domain of SNX25 reveals a novel phospholipid recognition model by dimerization in the PX domain
FEBS Lett., 591:2011-2018, 2017

PubMed Abstract: SNX25, a regulator of GPCR signaling-phox-homology (PX) domain containing sorting nexin (SNX) member, has been proposed to be involved in the lysosomal degradation of the transforming growth factor β receptor and the development of temporal lobe epilepsy. Targeting to the endosomal membranes by the specific binding of phosphorylated phosphatidylinositols (PIPs) through the PX domain is critical for the function of SNXs. However, the mechanism for SNX25-PX targeting to the endosomes remains unclear. Here, we demonstrate that the PX domain of zebrafish SNX25 (zSNX25-PX) is capable of binding to PI3P only in its dimeric form. We also present the crystal structure of zSNX25-PX. Combined with biochemical experiments, we further identify a potential PI3P-binding region and propose a novel PI-binding model based on dimerization in the PX domain of SNXs.

PubMed: 28542875
DOI: 10.1002/1873-3468.12688

Experimental method

X-RAY DIFFRACTION (1.7 Å)