5XDH

His/DOPA ligated cytochrome c from an anammox organism KSU-1

5XDH の概要

• エントリーDOI: 10.2210/pdb5xdh/pdb
• 分子名称: Putative cytochrome c, HEME C, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: cytochrome c, et protein, dopa ligand, electron transport
• 由来する生物種: Candidatus Jettenia caeni
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 40361.79

構造登録者

Hira, D.,Kitamura, R.,Nakamura, T.,Yamagata, Y.,Furukawa, K.,Fujii, T. (登録日: 2017-03-28, 公開日: 2018-03-28, 最終更新日: 2025-09-17)

主引用文献

Hira, D.,Kitamura, R.,Nakamura, T.,Yamagata, Y.,Furukawa, K.,Fujii, T.
Anammox Organism KSU-1 Expresses a Novel His/DOPA Ligated Cytochrome c.
J. Mol. Biol., 430:1189-1200, 2018

PubMed Abstract: Anammox is a bacterial energy metabolic process that forms N gas from nitrite and ammonium ions. The enzymatic mechanisms of anammox have been gradually revealed; however, the electron transport chain in anammox bacteria remains poorly understood. In the present study, we purified and characterized two low-molecular-weight c-type cytochromes from an enriched culture of the anammox bacterium strain, KSU-1. Their genes, KSU1_B0428 and KSU1_C0855, were identified in the KSU-1 genome, and their recombinant proteins were characterized. KSU1_B0428 is a typical c-type cytochrome with a His/Met coordinated heme, acting as an electron transfer protein. In contrast, KSU1_C0855 could not be assigned as a known cytochrome and its heme was suggested to have an uncommon axial ligand set. Crystal structural analyses of C0855 clearly showed that its heme iron is coordinated by His15 as a fifth ligand. Moreover, the sixth coordination site is occupied by the aromatic ring of Tyr60, and an unassignable electron density that is inseparable with that of aromatic carbon of Tyr60 was found. The additional electron density was assigned to an O atom by molecular mass analyses. Therefore, Tyr60 would be chemically modified to 3,4-dihydroxyphenylalanine and bound to the Fe atom. We revealed that an anammox bacterium strain KSU-1 expresses a novel cytochrome c having an unprecedented His/3,4-dihydroxyphenylalanine coordinating heme. The expression of the novel c-type cytochrome might be required for the redox reaction of the anammox process.

PubMed: 29481839
DOI: 10.1016/j.jmb.2018.02.017

実験手法

X-RAY DIFFRACTION (1.32 Å)