5XCZ

Structure of the cellobiohydrolase Cel6A from Phanerochaete chrysosporium in complex with cellobiose at 2.1 angstrom

5XCZ の概要

• エントリーDOI: 10.2210/pdb5xcz/pdb
• 関連するPDBエントリー: 5XCY
• 関連するBIRD辞書のPRD_ID: PRD_900023
• 分子名称: Glucanase, beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: glycosidehydrolase family 6, hydrolase
• 由来する生物種: Phanerochaete chrysosporium (White-rot fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38846.01

構造登録者

Tachioka, M.,Nakamura, A.,Ishida, T.,Igarashi, K.,Samejima, M. (登録日: 2017-03-24, 公開日: 2017-07-26, 最終更新日: 2024-11-13)

主引用文献

Tachioka, M.,Nakamura, A.,Ishida, T.,Igarashi, K.,Samejima, M.
Crystal structure of a family 6 cellobiohydrolase from the basidiomycete Phanerochaete chrysosporium
Acta Crystallogr F Struct Biol Commun, 73:398-403, 2017

PubMed Abstract: Cellobiohydrolases belonging to glycoside hydrolase family 6 (CBH II, Cel6A) play key roles in the hydrolysis of crystalline cellulose. CBH II from the white-rot fungus Phanerochaete chrysosporium (PcCel6A) consists of a catalytic domain (CD) and a carbohydrate-binding module connected by a linker peptide, like other known fungal cellobiohydrolases. In the present study, the CD of PcCel6A was crystallized without ligands, and p-nitrophenyl β-D-cellotrioside (pNPG3) was soaked into the crystals. The determined structures of the ligand-free and pNPG3-soaked crystals revealed that binding of cellobiose at substrate subsites +1 and +2 induces a conformational change of the N-terminal and C-terminal loops, switching the tunnel-shaped active site from the open to the closed form.

PubMed: 28695848
DOI: 10.1107/S2053230X17008093

実験手法

X-RAY DIFFRACTION (2.1 Å)