5XCT

Crystal structure of P20.1 Fv-clasp fragment with its antigen peptide

5XCT の概要

• エントリーDOI: 10.2210/pdb5xct/pdb
• 関連するPDBエントリー: 5XCQ 5XCR 5XCS 5XCT 5XCU 5XCX
• 分子名称: VH(S112C)-SARAH chimera, VL-SARAH(S37C)chimera, C8 peptide, ... (5 entities in total)
• 機能のキーワード: antibody fragment, fv-clasp, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 38084.55

構造登録者

Arimori, T.,Takagi, J. (登録日: 2017-03-23, 公開日: 2017-10-04, 最終更新日: 2024-10-16)

主引用文献

Arimori, T.,Kitago, Y.,Umitsu, M.,Fujii, Y.,Asaki, R.,Tamura-Kawakami, K.,Takagi, J.
Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability
Structure, 25:1611-1622.e4, 2017

PubMed Abstract: Antibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this particular application. By fusing an anti-parallel coiled-coil structure derived from the SARAH domain of human Mst1 kinase to the variable region of an antibody, we succeeded in creating a novel chimeric antibody fragment of ∼37 kDa, termed "Fv-clasp," which exhibits excellent crystallization compatibility while maintaining the binding ability of the original IgG molecule. The "clasp" and the engineered disulfide bond at the bottom of the Fv suppressed the internal mobility of the fragment and shielded hydrophobic residues, likely contributing to the high heat stability and the crystallizability of the Fv-clasp. Finally, Fv-clasp antibodies showed superior "chaperoning" activity over conventional Fab fragments, and facilitated the structure determination of an ectodomain fragment of integrin α6β1.

PubMed: 28919443
DOI: 10.1016/j.str.2017.08.011

実験手法

X-RAY DIFFRACTION (1.17 Å)