5X6G

Crystal Structure of SMAD5-MH1/palindromic SBE DNA complex

「4ZKG」から置き換えられました

5X6G の概要

• エントリーDOI: 10.2210/pdb5x6g/pdb
• 関連するPDBエントリー: 5X6H 5X6M
• 分子名称: Mothers against decapentaplegic homolog 5, DNA (5'-D(P*AP*TP*CP*AP*GP*TP*CP*TP*AP*GP*AP*CP*AP*TP*A)-3'), DNA (5'-D(P*GP*TP*AP*TP*GP*TP*CP*TP*AP*GP*AP*CP*TP*GP*A)-3'), ... (4 entities in total)
• 機能のキーワード: smad5, mh1 domain, protein-dna complex, sbe, metal binding protein-dna complex, metal binding protein/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Cytoplasm : P97454
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44569.69

構造登録者

Chai, N.,Wang, J.,Wang, Z.X.,Wu, J.W. (登録日: 2017-02-21, 公開日: 2017-03-15, 最終更新日: 2023-11-22)

主引用文献

Chai, N.,Li, W.X.,Wang, J.,Wang, Z.X.,Yang, S.M.,Wu, J.W.
Structural basis for the Smad5 MH1 domain to recognize different DNA sequences.
Nucleic Acids Res., 43:9051-9064, 2015

PubMed Abstract: Smad proteins are important intracellular mediators of TGF-β signalling, which transmit signals directly from cell surface receptors to the nucleus. The MH1 domain of Smad plays a key role in DNA recognition. Two types of DNA sequence were identified as Smad binding motifs: the Smad binding element (SBE) and the GC-rich sequence. Here we report the first crystal structure of the Smad5 MH1 domain in complex with the GC-rich sequence. Compared with the Smad5-MH1/SBE complex structure, the Smad5 MH1 domain contacts the GC-rich site with the same β-hairpin, but the detailed interaction modes are different. Conserved β-hairpin residues make base specific contacts with the minimal GC-rich site, 5'-GGC-3'. The assembly of Smad5-MH1 on the GC-rich DNA also results in distinct DNA conformational changes. Moreover, the crystal structure of Smad5-MH1 in complex with a composite DNA sequence demonstrates that the MH1 domain is targeted to each binding site (GC-rich or SBE) with modular binding modes, and the length of the DNA spacer affects the MH1 assembly. In conclusion, our work provides the structural basis for the recognition and binding specificity of the Smad MH1 domain with the DNA targets.

PubMed: 26304548
DOI: 10.1093/nar/gkv848

実験手法

X-RAY DIFFRACTION (3.05 Å)