5X56

Crystal structure of PSB27 from Arabidopsis thaliana

Summary for 5X56

• Entry DOI: 10.2210/pdb5x56/pdb
• Descriptor: Photosystem II repair protein PSB27-H1, chloroplastic (2 entities in total)
• Functional Keywords: photosynthesis protein, photosynthesis
• Biological source: Arabidopsis thaliana (Mouse-ear cress)
• Cellular location: Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein ; Lumenal side : Q9LR64
• Total number of polymer chains: 2
• Total formula weight: 23442.57

Authors

Liu, J.,Cheng, X. (deposition date: 2017-02-15, release date: 2017-12-27, Last modification date: 2023-11-22)

Primary citation

Cheng, X.,Liu, J.,Huan, Z.,Fudong, L.,Shuya, Z.,Min, X.,Ke, R.,Yuhua, W.,Aigen, F.
Crystal structure of Psb27 from Arabidopsis thaliana determined at a resolution of 1.85 angstrom.
Photosyn. Res., 136:139-146, 2018

PubMed Abstract: Proper biogenesis and maintenance of photosynthetic thylakoid membrane complexes are essential for the photosynthetic light reactions. A thylakoid lumenal protein, Psb27, plays a vital role in assembly or/and maintenance of photosystem II (PSII). In cyanobacteria, it is a small lipoprotein docked to the lumenal side of PSII, and functions in the assembly of the MnCa cluster and in the PSII repair cycle. However, Psb27 from Arabidopsis thaliana is not a lipoprotein, and it is involved in PSII repair and acclimation to fluctuating light stress, suggesting a functional divergence between Arabidopsis Psb27 and cyanobacterial Psb27s. To gain a better understanding of Psb27 from higher plants, we determined the crystal structure of Arabidopsis Psb27 by X-ray crystallography at a resolution of 1.85 Å. The structure of Arabidopsis Psb27 is a four-helix bundle, similar to its orthologues from cyanobacteria. However, there are several structural differences between Arabidopsis Psb27 and cyanobacterial Psb27s concerning the overall molecular shape, the N- and C-terminal structures, and the surface charge. These differences suggest that Psb27 from higher plants and cyanobacteria may function differently.

PubMed: 29098572
DOI: 10.1007/s11120-017-0450-3

Experimental method

X-RAY DIFFRACTION (1.85 Å)