5X31

Pseudoazurin from Alcaligenes faecalis (space group P65)

5X31 の概要

• エントリーDOI: 10.2210/pdb5x31/pdb
• 分子名称: Pseudoazurin, COPPER (II) ION (3 entities in total)
• 機能のキーワード: cupredoxin, pseudoazurin, copper, electron transfer, electron transport
• 由来する生物種: Alcaligenes faecalis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27472.82

構造登録者

Fukuda, Y.,Mizohata, E.,Inoue, T. (登録日: 2017-02-03, 公開日: 2017-04-12, 最終更新日: 2023-11-22)

主引用文献

Fukuda, Y.,Mizohata, E.,Inoue, T.
New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper
Acta Crystallogr F Struct Biol Commun, 73:159-166, 2017

PubMed Abstract: Pseudoazurin from the denitrifying bacterium Alcaligenes faecalis (AfPAz) is a blue copper protein and functions as an electron donor to copper-containing nitrite reductase (CuNIR). Conventionally, AfPAz has been crystallized using highly concentrated ammonium sulfate as a precipitant. Here, a needle-like crystal of AfPAz grown in a solution containing a macromolecular precipitant, polyethylene glycol 8000 (PEG 8000), is reported. The crystal belonged to space group P6, with unit-cell parameters a = b = 68.7, c = 94.2 Å. The structure has been determined and refined at 2.6 Å resolution. The asymmetric unit contained two AfPAz molecules contacting each other on negatively charged surfaces. The molecular packing of the crystal showed a right-handed double-helical arrangement of AfPAz molecules and hence of blue copper sites. This structure provides insight into the excluded-volume effect of PEG and the manner of assembly of AfPAz.

PubMed: 28291752
DOI: 10.1107/S2053230X17002631

実験手法

X-RAY DIFFRACTION (2.6 Å)