5WXZ

Crystal structure of Microcystis aeruginosa PCC 7806 aspartate racemase in complex with D-aspartate

5WXZ の概要

• エントリーDOI: 10.2210/pdb5wxz/pdb
• 関連するPDBエントリー: 5WXX 5WXY
• 分子名称: McyF, D-ASPARTIC ACID (3 entities in total)
• 機能のキーワード: aspartate racemase, mcyf, microcystin, isomerase
• 由来する生物種: Microcystis aeruginosa PCC 7806
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29346.71

構造登録者

Cao, D.D.,Zhou, K.,Jiang, Y.L.,Zhou, C.Z. (登録日: 2017-01-09, 公開日: 2018-01-17, 最終更新日: 2023-11-22)

主引用文献

Cao, D.D.,Zhang, C.P.,Zhou, K.,Jiang, Y.L.,Tan, X.F.,Xie, J.,Ren, Y.M.,Chen, Y.,Zhou, C.Z.,Hou, W.T.
Structural insights into the catalysis and substrate specificity of cyanobacterial aspartate racemase McyF.
Biochem.Biophys.Res.Commun., 514:1108-1114, 2019

PubMed Abstract: L-amino acids represent the most common amino acid form, most notably as protein residues, whereas D-amino acids, despite their rare occurrence, play significant roles in many biological processes. Amino acid racemases are enzymes that catalyze the interconversion of L- and/or D-amino acids. McyF is a pyridoxal 5'-phosphate (PLP) independent amino acid racemase that produces the substrate D-aspartate for the biosynthesis of microcystin in the cyanobacterium Microcystis aeruginosa PCC7806. Here we report the crystal structures of McyF in complex with citrate, L-Asp and D-Asp at 2.35, 2.63 and 2.80 Å, respectively. Structural analyses indicate that McyF and homologs possess highly conserved residues involved in substrate binding and catalysis. In addition, residues Cys87 and Cys195 were clearly assigned to the key catalytic residues of "two bases" that deprotonate D-Asp and L-Asp in a reaction independent of PLP. Further site-directed mutagenesis combined with enzymatic assays revealed that Glu197 also participates in the catalytic reaction. In addition, activity assays proved that McyF could also catalyze the interconversion of L-MeAsp between D-MeAsp, the precursor of another microcystin isoform. These findings provide structural insights into the catalytic mechanism of aspartate racemase and microcystin biosynthesis.

PubMed: 31101340
DOI: 10.1016/j.bbrc.2019.05.063

実験手法

X-RAY DIFFRACTION (2.8 Å)