5WXM
Crystal structure of the Imp3 and Mpp10 complex
Summary for 5WXM
| Entry DOI | 10.2210/pdb5wxm/pdb |
| Descriptor | U3 small nucleolar ribonucleoprotein protein IMP3, U3 small nucleolar RNA-associated protein MPP10, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | protein complex, components of 90s preribosome, ribosomal protein |
| Biological source | Saccharomyces cerevisiae S288c More |
| Cellular location | Nucleus, nucleolus : P32899 P47083 |
| Total number of polymer chains | 4 |
| Total formula weight | 46099.46 |
| Authors | |
| Primary citation | Sun, Q.,Zhu, X.,Qi, J.,An, W.,Lan, P.,Tan, D.,Chen, R.,Wang, B.,Zheng, S.,Zhang, C.,Chen, X.,Zhang, W.,Chen, J.,Dong, M.Q.,Ye, K. Molecular architecture of the 90S small subunit pre-ribosome Elife, 6:-, 2017 Cited by PubMed Abstract: Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors. PubMed: 28244370DOI: 10.7554/eLife.22086 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.304 Å) |
Structure validation
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