5WXM

Crystal structure of the Imp3 and Mpp10 complex

5WXM の概要

• エントリーDOI: 10.2210/pdb5wxm/pdb
• 分子名称: U3 small nucleolar ribonucleoprotein protein IMP3, U3 small nucleolar RNA-associated protein MPP10, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: protein complex, components of 90s preribosome, ribosomal protein
• 由来する生物種: Saccharomyces cerevisiae S288c; 詳細
• 細胞内の位置: Nucleus, nucleolus : P32899 P47083
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 46099.46

構造登録者

Ye, K.,Zheng, S. (登録日: 2017-01-07, 公開日: 2017-06-28, 最終更新日: 2024-11-20)

主引用文献

Sun, Q.,Zhu, X.,Qi, J.,An, W.,Lan, P.,Tan, D.,Chen, R.,Wang, B.,Zheng, S.,Zhang, C.,Chen, X.,Zhang, W.,Chen, J.,Dong, M.Q.,Ye, K.
Molecular architecture of the 90S small subunit pre-ribosome
Elife, 6:-, 2017

PubMed Abstract: Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.

PubMed: 28244370
DOI: 10.7554/eLife.22086

実験手法

X-RAY DIFFRACTION (2.304 Å)