5WWE

Crystal structure of hnRNPA2B1 in complex with RNA

5WWE の概要

• エントリーDOI: 10.2210/pdb5wwe/pdb
• 関連するPDBエントリー: 5WWF 5WWG 5WWH
• 分子名称: Heterogeneous nuclear ribonucleoproteins A2/B1, RNA (5'-R(*AP*GP*GP*GP*AP*CP*UP*AP*GP*A)-3') (3 entities in total)
• 機能のキーワード: hnrnp, rrm, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus, nucleoplasm . Isoform A2: Nucleus : P22626
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24446.98

構造登録者

Wu, B.X.,Su, S.C.,Ma, J.B. (登録日: 2017-01-01, 公開日: 2018-01-17, 最終更新日: 2023-11-22)

主引用文献

Wu, B.,Su, S.,Patil, D.P.,Liu, H.,Gan, J.,Jaffrey, S.R.,Ma, J.
Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1.
Nat Commun, 9:420-420, 2018

PubMed Abstract: Human hnRNP A2/B1 is an RNA-binding protein that plays important roles in many biological processes, including maturation, transport, and metabolism of mRNA, and gene regulation of long noncoding RNAs. hnRNP A2/B1 was reported to control the microRNAs sorting to exosomes and promote primary microRNA processing as a potential mA "reader." hnRNP A2/B1 contains two RNA recognition motifs that provide sequence-specific recognition of RNA substrates. Here, we determine crystal structures of tandem RRM domains of hnRNP A2/B1 in complex with various RNA substrates, elucidating specific recognitions of AGG and UAG motifs by RRM1 and RRM2 domains, respectively. Further structural and biochemical results demonstrate multivariant binding modes for sequence-diversified RNA substrates, supporting a RNA matchmaker mechanism in hnRNP A2/B1 function. Moreover, our studies in combination with bioinformatic analysis suggest that hnRNP A2/B1 may mediate effects of mA through a "mA switch" mechanism, instead of acting as a direct "reader" of mA modification.

PubMed: 29379020
DOI: 10.1038/s41467-017-02770-z

実験手法

X-RAY DIFFRACTION (2.4 Å)