5WVB

Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)6 (CAD1-E217L-(GlcNAc)6 ) from Ostrinia furnacalis

5WVB の概要

• エントリーDOI: 10.2210/pdb5wvb/pdb
• 関連するPDBエントリー: 5WV8 5WV9 5WVF 5WVG 5WVH
• 分子名称: Chitinase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: ostrinia furnacalis, chitinase, three-dimensional structure, chitin metabolism, hydrolase, (glcnac)6
• 由来する生物種: Ostrinia furnacalis (Asian corn borer)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55263.54

構造登録者

Liu, T.,Zhou, Y.,Yang, Q. (登録日: 2016-12-23, 公開日: 2017-12-27, 最終更新日: 2024-11-13)

主引用文献

Liu, T.,Zhu, W.,Wang, J.,Zhou, Y.,Duan, Y.,Qu, M.,Yang, Q.
The deduced role of a chitinase containing two nonsynergistic catalytic domains
Acta Crystallogr D Struct Biol, 74:30-40, 2018

PubMed Abstract: The glycoside hydrolase family 18 chitinases degrade or alter chitin. Multiple catalytic domains in a glycoside hydrolase family 18 chitinase function synergistically during chitin degradation. Here, an insect group III chitinase from the agricultural pest Ostrinia furnacalis (OfChtIII) is revealed to be an arthropod-conserved chitinase that contains two nonsynergistic GH18 domains according to its catalytic properties. Both GH18 domains are active towards single-chained chitin substrates, but are inactive towards insoluble chitin substrates. The crystal structures of each unbound GH18 domain, as well as of GH18 domains complexed with hexa-N-acetyl-chitohexaose or penta-N-acetyl-chitopentaose, suggest that the two GH18 domains possess endo-specific activities. Physiological data indicated that the developmental stage-dependent gene-expression pattern of OfChtIII was the same as that of the chitin synthase OfChsA but significantly different from that of the chitinase OfChtI, which is indispensable for cuticular chitin degradation. Additionally, immunological staining indicated that OfChtIII was co-localized with OfChsA. Thus, OfChtIII is most likely to be involved in the chitin-synthesis pathway.

PubMed: 29372897
DOI: 10.1107/S2059798317018289

実験手法

X-RAY DIFFRACTION (3.099 Å)