5WTX

Structure of Nop9

5WTX の概要

• エントリーDOI: 10.2210/pdb5wtx/pdb
• 関連するPDBエントリー: 5WTY
• 分子名称: Nucleolar protein 9 (1 entity in total)
• 機能のキーワード: pumilio repeat-containing protein, rna-binding protein, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae S288c
• 細胞内の位置: Nucleus, nucleolus : P47077
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 77839.15

構造登録者

Ye, K.,Wang, B. (登録日: 2016-12-15, 公開日: 2017-01-25, 最終更新日: 2024-03-20)

主引用文献

Wang, B.,Ye, K.
Nop9 binds the central pseudoknot region of 18S rRNA
Nucleic Acids Res., 45:3559-3567, 2017

PubMed Abstract: The assembly of eukaryotic ribosomes requires numerous factors that transiently associate with evolving pre-ribosomal particles. The Pumilio repeat-containing protein Nop9 briefly associates with the 90S pre-ribosome during its co-transcriptional assembly. Here, we show that Nop9 specifically binds an 11-nucleotide sequence of 18S rRNA that forms the 3΄ side of the central pseudoknot and helix 28 in the mature subunit. Crystal structures of Nop9 in the free and RNA-bound states reveal a new type of Pumilio repeat protein with a distinct structure, target sequence and RNA-binding mode. Nop9 contains 10 Pumilio repeats arranged into a U-shaped scaffold. The target RNA is recognized by two stretches of repeats in a bipartite manner, and three central bases are unrecognized as a result of the degeneracy of repeats 6 and 7. Our data suggest that Nop9 regulates the folding of 18S rRNA at early assembly stages of 90S.

PubMed: 28053123
DOI: 10.1093/nar/gkw1323

実験手法

X-RAY DIFFRACTION (3.074 Å)