5WTH

Cryo-EM structure for Hepatitis A virus complexed with FAB

5WTH の概要

• エントリーDOI: 10.2210/pdb5wth/pdb
• 関連するPDBエントリー: 5WTE 5WTF
• EMDBエントリー: 6686 6687 6688
• 分子名称: Polyprotein, VP2, VP3, ... (5 entities in total)
• 機能のキーワード: hav, neutralizing mechanism, receptor recognition, viral entry, virus
• 由来する生物種: Hepatovirus A; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 130512.68

構造登録者

Wang, X.,Zhu, L.,Dang, M.,Hu, Z.,Gao, Q.,Yuan, S.,Sun, Y.,Zhang, B.,Ren, J.,Walter, T.S.,Wang, J.,Fry, E.E.,Stuart, D.I.,Rao, Z. (登録日: 2016-12-12, 公開日: 2017-01-25, 最終更新日: 2024-10-23)

主引用文献

Wang, X.,Zhu, L.,Dang, M.,Hu, Z.,Gao, Q.,Yuan, S.,Sun, Y.,Zhang, B.,Ren, J.,Kotecha, A.,Walter, T.S.,Wang, J.,Fry, E.E.,Stuart, D.I.,Rao, Z.
Potent neutralization of hepatitis A virus reveals a receptor mimic mechanism and the receptor recognition site
Proc. Natl. Acad. Sci. U.S.A., 114:770-775, 2017

PubMed Abstract: Hepatitis A virus (HAV) infects ∼1.4 million people annually and, although there is a vaccine, there are no licensed therapeutic drugs. HAV is unusually stable (making disinfection problematic) and little is known of how it enters cells and releases its RNA. Here we report a potent HAV-specific monoclonal antibody, R10, which neutralizes HAV infection by blocking attachment to the host cell. High-resolution cryo-EM structures of HAV full and empty particles and of the complex of HAV with R10 Fab reveal the atomic details of antibody binding and point to a receptor recognition site at the pentamer interface. These results, together with our observation that the R10 Fab destabilizes the capsid, suggest the use of a receptor mimic mechanism to neutralize virus infection, providing new opportunities for therapeutic intervention.

PubMed: 28074040
DOI: 10.1073/pnas.1616502114

実験手法

ELECTRON MICROSCOPY (4.2 Å)