5WRT

Crystal structure of type I inorganic pyrophosphatase from Toxoplasma gondii.

5WRT の概要

• エントリーDOI: 10.2210/pdb5wrt/pdb
• 関連するPDBエントリー: 5WRU
• 分子名称: Soluble inorganic pyrophosphatase, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: ppi, ppase, novel interface, hydrolase
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53670.32

構造登録者

Jamwal, A.,Yogavel, M.,Sharma, A. (登録日: 2016-12-03, 公開日: 2017-10-11, 最終更新日: 2024-03-20)

主引用文献

Jamwal, A.,Yogavel, M.,Abdin, M.Z.,Jain, S.K.,Sharma, A.
Structural and Biochemical Characterization of Apicomplexan Inorganic Pyrophosphatases
Sci Rep, 7:5255-5255, 2017

PubMed Abstract: Inorganic pyrophosphatases (PPase) participate in energy cycling and they are essential for growth and survival of organisms. Here we report extensive structural and functional characterization of soluble PPases from the human parasites Plasmodium falciparum (PfPPase) and Toxoplasma gondii (TgPPase). Our results show that PfPPase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages. Cambialistic PfPPase actively hydrolyzes linear short chain polyphosphates like PP, polyP and ATP in the presence of Zn. A remarkable new feature of PfPPase is the low complexity asparagine-rich N-terminal region that mediates its dimerization. Deletion of N-region has an unexpected and substantial effect on the stability of PfPPase domain, resulting in aggregation and significant loss of enzyme activity. Significantly, the crystal structures of PfPPase and TgPPase reveal unusual and unprecedented dimeric organizations and provide new fundamental insights into the variety of oligomeric assemblies possible in eukaryotic inorganic PPases.

PubMed: 28701714
DOI: 10.1038/s41598-017-05234-y

実験手法

X-RAY DIFFRACTION (2.348 Å)