5WRB
Crystal structure of hen egg-white lysozyme
Summary for 5WRB
| Entry DOI | 10.2210/pdb5wrb/pdb |
| Related | 5WR9 5WRA |
| Descriptor | Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | xfel, sfx, hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Cellular location | Secreted: P00698 |
| Total number of polymer chains | 1 |
| Total formula weight | 14389.60 |
| Authors | Sugahara, M.,Suzuki, M.,Masuda, T.,Inoue, S.,Nango, E. (deposition date: 2016-12-01, release date: 2017-12-20, Last modification date: 2024-10-30) |
| Primary citation | Sugahara, M.,Nakane, T.,Masuda, T.,Suzuki, M.,Inoue, S.,Song, C.,Tanaka, R.,Nakatsu, T.,Mizohata, E.,Yumoto, F.,Tono, K.,Joti, Y.,Kameshima, T.,Hatsui, T.,Yabashi, M.,Nureki, O.,Numata, K.,Nango, E.,Iwata, S. Hydroxyethyl cellulose matrix applied to serial crystallography Sci Rep, 7:703-703, 2017 Cited by PubMed Abstract: Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images. PubMed: 28386083DOI: 10.1038/s41598-017-00761-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.013 Å) |
Structure validation
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