5WQV

Crystal structure of PriB mutant - S55A

5WQV の概要

• エントリーDOI: 10.2210/pdb5wqv/pdb
• 分子名称: Primosomal replication protein N (2 entities in total)
• 機能のキーワード: dna binding protein, bacterial dna replication, ob-fold, s55a mutant
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22886.39

構造登録者

Fujiyama, S.,Shiroishi, M.,Katayama, T.,Abe, Y.,Ueda, T. (登録日: 2016-11-28, 公開日: 2017-11-29, 最終更新日: 2024-11-06)

主引用文献

Fujiyama, S.,Abe, Y.,Shiroishi, M.,Ikeda, Y.,Ueda, T.
Insight into the interaction between PriB and DnaT on bacterial DNA replication restart: Significance of the residues on PriB dimer interface and highly acidic region on DnaT.
Biochim Biophys Acta Proteins Proteom, 1867:367-375, 2019

PubMed Abstract: When the replisome collapses at a DNA damage site, a sequence-independent replication restart system is required. In Escherichia coli, PriA, PriB, and DnaT assemble in an orderly fashion at the stalled replication fork and achieve the reloading of the replisome. PriB-DnaT interaction is considered a significant step in the replication restart. In this study, we examined the contribution of the residues Ser20, His26 and Ser55, which are located on the PriB dimer interface. These residues are proximal to Glu39 and Arg44, which are important for PriB-DnaT interaction. Mutational analyses revealed that His26 and Ser20 of PriB are important for the interaction with DnaT, and that the Ser55 residue of PriB might have a role in negatively regulating the DnaT binding. These residues are involved in not only the interaction between PriB and DnaT but also the dissociation of single-stranded DNA (ssDNA) from the PriB-ssDNA complex due to DnaT binding. Moreover, NMR study indicates that the region Asp66-Glu76 on the linker between DnaT domains is involved in the interaction with wild-type PriB. These findings provide significant information about the molecular mechanism underlying replication restart in bacteria.

PubMed: 30659961
DOI: 10.1016/j.bbapap.2019.01.008

実験手法

X-RAY DIFFRACTION (1.97 Å)