5WPW

Crystal structure of coconut allergen cocosin

5WPW の概要

• エントリーDOI: 10.2210/pdb5wpw/pdb
• 分子名称: 11S globulin isoform 1 (2 entities in total)
• 機能のキーワード: 11s globulin, seed storage protein, allergen
• 由来する生物種: Cocos nucifera (Coconut palm)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96356.53

構造登録者

Jin, T.,Zhang, Y. (登録日: 2016-11-21, 公開日: 2017-10-25, 最終更新日: 2024-10-23)

主引用文献

Jin, T.,Wang, C.,Zhang, C.,Wang, Y.,Chen, Y.W.,Guo, F.,Howard, A.,Cao, M.J.,Fu, T.J.,McHugh, T.H.,Zhang, Y.
Crystal Structure of Cocosin, A Potential Food Allergen from Coconut (Cocos nucifera)
J. Agric. Food Chem., 65:7560-7568, 2017

PubMed Abstract: Coconut (Cocos nucifera) is an important palm tree. Coconut fruit is widely consumed. The most abundant storage protein in coconut fruit is cocosin (a likely food allergen), which belongs to the 11S globulin family. Cocosin was crystallized near a century ago, but its structure remains unknown. By optimizing crystallization conditions and cryoprotectant solutions, we were able to obtain cocosin crystals that diffracted to 1.85 Å. The cocosin gene was cloned from genomic DNA isolated from dry coconut tissue. The protein sequence deduced from the predicted cocosin coding sequence was used to guide model building and structure refinement. The structure of cocosin was determined for the first time, and it revealed a typical 11S globulin feature of a double layer doughnut-shaped hexamer.

PubMed: 28712292
DOI: 10.1021/acs.jafc.7b02252

実験手法

X-RAY DIFFRACTION (1.847 Å)