5WMO

Crystal Structure of HLA-B7 in complex with RPP, an EBV peptide

5WMO の概要

• エントリーDOI: 10.2210/pdb5wmo/pdb
• 分子名称: HLA class I histocompatibility antigen, B-7 alpha chain, Beta-2-microglobulin, RPP peptide from EBV, ... (4 entities in total)
• 機能のキーワード: hla, viral peptide, iav, ebv, cmv, crossreactivity, tcr, t cell, heterologous immunity, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45049.87

構造登録者

Gras, S.,Rossjohn, J. (登録日: 2017-07-30, 公開日: 2018-06-06, 最終更新日: 2024-10-09)

主引用文献

Rowntree, L.C.,Nguyen, T.H.O.,Halim, H.,Purcell, A.W.,Rossjohn, J.,Gras, S.,Kotsimbos, T.C.,Mifsud, N.A.
Inability To Detect Cross-Reactive Memory T Cells Challenges the Frequency of Heterologous Immunity among Common Viruses.
J. Immunol., 200:3993-4003, 2018

PubMed Abstract: Human memory T cells that cross-react with epitopes from unrelated viruses can potentially modulate immune responses to subsequent infections by a phenomenon termed heterologous immunity. However, it is unclear whether similarities in structure rather than sequence underpin heterologous T cell cross-reactivity. In this study, we aimed to explore the mechanism of heterologous immunity involving immunodominant epitopes derived from common viruses restricted to high-frequency HLA allotypes (HLA-A*02:01, -B*07:02, and -B*08:01). We examined EBV-specific memory T cells for their ability to cross-react with CMV or influenza A virus-derived epitopes. Following T cell immunoassays to determine phenotype and function, complemented with biophysical and structural investigations of peptide/HLA complexes, we did not detect cross-reactivity of EBV-specific memory T cells toward either CMV or influenza A virus epitopes presented by any of the selected HLA allomorphs. Thus, despite the ubiquitous nature of these human viruses and the dominant immune response directed toward the selected epitopes, heterologous virus-specific T cell cross-reactivity was not detected. This suggests that either heterologous immunity is not as common as previously reported, or that it requires a very specific biological context to develop and be clinically relevant.

PubMed: 29735483
DOI: 10.4049/jimmunol.1800010

実験手法

X-RAY DIFFRACTION (1.62 Å)