5WM2

Crystal Structure of CahJ in Complex with Salicylic Acid and AMP

5WM2 の概要

• エントリーDOI: 10.2210/pdb5wm2/pdb
• 分子名称: Salicylate-AMP ligase, 2-HYDROXYBENZOIC ACID, ADENOSINE MONOPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: ligase, adenylation domain, peptide synthetase
• 由来する生物種: Streptomyces gandocaensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61512.10

構造登録者

Sikkema, A.P.,Smith, J.L. (登録日: 2017-07-28, 公開日: 2018-05-23, 最終更新日: 2023-10-04)

主引用文献

Tripathi, A.,Park, S.R.,Sikkema, A.P.,Cho, H.J.,Wu, J.,Lee, B.,Xi, C.,Smith, J.L.,Sherman, D.H.
A Defined and Flexible Pocket Explains Aryl Substrate Promiscuity of the Cahuitamycin Starter Unit-Activating Enzyme CahJ.
Chembiochem, 19:1595-1600, 2018

PubMed Abstract: Cahuitamycins are biofilm inhibitors assembled by a convergent nonribosomal peptide synthetase pathway. Previous genetic analysis indicated that a discrete enzyme, CahJ, serves as a gatekeeper for cahuitamycin structural diversification. Here, the CahJ protein was probed structurally and functionally to guide the formation of new analogues by mutasynthetic studies. This analysis enabled the in vivo production of a new cahuitamycin congener through targeted precursor incorporation.

PubMed: 29742306
DOI: 10.1002/cbic.201800233

実験手法

X-RAY DIFFRACTION (1.548 Å)